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cd34 progenitor cells selection system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cd34 progenitor cells selection system
    A. A representative histogram and dot plots showing <t>CD34-expression</t> after purification from cord blood depicted as fluorescence intensity values and percentage of <t>CD34+</t> cells compared to the corresponding isotype control, respectively. B. The morphology and culture procedure for CD34+ cells after purification, seven days in expansion media, three days in medium and another three days in EGM-2 on fibronectin coated dishes. The fold expansion of cells represent mean and SD of n = 9.
    Cd34 Progenitor Cells Selection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+progenitor+cell+selection+system/pmc03155543-43-15-20
    Average 90 stars, based on 1 article reviews
    cd34 progenitor cells selection system - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Successful In Vitro Expansion and Differentiation of Cord Blood Derived CD34+ Cells into Early Endothelial Progenitor Cells Reveals Highly Differential Gene Expression"

    Article Title: Successful In Vitro Expansion and Differentiation of Cord Blood Derived CD34+ Cells into Early Endothelial Progenitor Cells Reveals Highly Differential Gene Expression

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0023210

    A. A representative histogram and dot plots showing CD34-expression after purification from cord blood depicted as fluorescence intensity values and percentage of CD34+ cells compared to the corresponding isotype control, respectively. B. The morphology and culture procedure for CD34+ cells after purification, seven days in expansion media, three days in medium and another three days in EGM-2 on fibronectin coated dishes. The fold expansion of cells represent mean and SD of n = 9.
    Figure Legend Snippet: A. A representative histogram and dot plots showing CD34-expression after purification from cord blood depicted as fluorescence intensity values and percentage of CD34+ cells compared to the corresponding isotype control, respectively. B. The morphology and culture procedure for CD34+ cells after purification, seven days in expansion media, three days in medium and another three days in EGM-2 on fibronectin coated dishes. The fold expansion of cells represent mean and SD of n = 9.

    Techniques Used: Expressing, Purification, Fluorescence

    A. Binding of ulex-lectin and uptake of acetylated LDL (AcLDL) on EPCs visualised with fluorescence microscopy and flow cytometry using FITC-labelled ulex-lectin and Dil-labelled acetylated LDL, respectively. B. Summary of median fluorescence intensity for CD34-FITC, CD61-FITC, CD144-PE, CD309-PE, CD45-FITC and CD18-FITC of five donors expressed as mean and SD compensated for respective isotype control. C. Representative histograms comparing fluorescence intensity on early EPCs and HUVECs of the cell surface markers for CD34, CD61, CD144, CD309, CD45 and CD18 (blue line) and corresponding isotype control (red line).
    Figure Legend Snippet: A. Binding of ulex-lectin and uptake of acetylated LDL (AcLDL) on EPCs visualised with fluorescence microscopy and flow cytometry using FITC-labelled ulex-lectin and Dil-labelled acetylated LDL, respectively. B. Summary of median fluorescence intensity for CD34-FITC, CD61-FITC, CD144-PE, CD309-PE, CD45-FITC and CD18-FITC of five donors expressed as mean and SD compensated for respective isotype control. C. Representative histograms comparing fluorescence intensity on early EPCs and HUVECs of the cell surface markers for CD34, CD61, CD144, CD309, CD45 and CD18 (blue line) and corresponding isotype control (red line).

    Techniques Used: Binding Assay, Fluorescence, Microscopy, Flow Cytometry

    A. A representative colony forming unit (CFU-Hill) visualised with giemsa staining and characterised with binding of FITC-labelled ulex-lectin and uptake of Dil-labelled acetylated LDL. B. Representative images of capillary network formed in a endothelial tube formation matrigel assay by HUVECs alone or HUVECs co-cultured with early EPCs. C Early EPCs pre-stained with cell tracker green (arrows) traced along the tubuli and in the branching area of capillary network D. The bar graph shows the total number of branching points (mean and SD, n = 3) and the box plot show the length of tubuli (dotted line represents the mean and the solid line represents the median, n = 488) in the endothelial tube formation matrigel with HUVECs alone or HUVECs co-cultured with early EPCs.
    Figure Legend Snippet: A. A representative colony forming unit (CFU-Hill) visualised with giemsa staining and characterised with binding of FITC-labelled ulex-lectin and uptake of Dil-labelled acetylated LDL. B. Representative images of capillary network formed in a endothelial tube formation matrigel assay by HUVECs alone or HUVECs co-cultured with early EPCs. C Early EPCs pre-stained with cell tracker green (arrows) traced along the tubuli and in the branching area of capillary network D. The bar graph shows the total number of branching points (mean and SD, n = 3) and the box plot show the length of tubuli (dotted line represents the mean and the solid line represents the median, n = 488) in the endothelial tube formation matrigel with HUVECs alone or HUVECs co-cultured with early EPCs.

    Techniques Used: Staining, Binding Assay, Matrigel Assay, Cell Culture

    Related Articles

    Purification:

    Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice
    Article Snippet: .. CD34 cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal CD34 progenitor cell selection system (Invitrogen) according to the manufacturer’s instructions. ..

    Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice
    Article Snippet: .. CD34 + cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal CD34 progenitor cell selection system (Invitrogen) according to the manufacturer's instructions. ..

    Derivative Assay:

    Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice
    Article Snippet: .. CD34 cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal CD34 progenitor cell selection system (Invitrogen) according to the manufacturer’s instructions. ..

    Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice
    Article Snippet: .. CD34 + cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal CD34 progenitor cell selection system (Invitrogen) according to the manufacturer's instructions. ..

    FACS:

    Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice
    Article Snippet: .. CD34 cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal CD34 progenitor cell selection system (Invitrogen) according to the manufacturer’s instructions. ..

    Article Title: CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex
    Article Snippet: .. Thymocyte suspensions were subjected to density centrifugation using Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS), and Early T-cell progenitors (ETPs) were then isolated by immunomagnetic cell sorting using the Dynal CD34 Progenitor Cell Selection System (Life Technologies). ..

    Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice
    Article Snippet: .. CD34 + cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal CD34 progenitor cell selection system (Invitrogen) according to the manufacturer's instructions. ..

    Selection:

    Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice
    Article Snippet: .. CD34 cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal CD34 progenitor cell selection system (Invitrogen) according to the manufacturer’s instructions. ..

    Article Title: Biochemical and Parasitological Studies on the Effect of hUCB-Selected CD34 + Progenitor/Stem Cells in Mice Infected with Schistosoma mansoni
    Article Snippet: After Ficoll-paque gradient separation, collected hUCB MNCs were washed twice in Dulbecco’s PBS. .. Isolation of the positively selected CD34 cells was then carried out using immunomagnetic separation technique by immune beads of Dynal CD34 + progenitor cell selection system (MACS ® ) following the procedure protocol according to the manufacturer’s recommendations. ..

    Article Title: The NOTCH1/CD44 axis drives pathogenesis in a T cell acute lymphoblastic leukemia model
    Article Snippet: .. ETPs were isolated as described ( 19 ) from thymocyte suspensions obtained by centrifugation on Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS) using the Dynal CD34 Progenitor Cell Selection System (Life Technologies). .. HSPCs were obtained from Ficoll-Hypaque–purified CB samples by AutoMACS immunomagnetic sorting using the CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec).

    Article Title: The C-C motif chemokine ligands CCL5, CCL11, and CCL24 induce the migration of circulating fibrocytes from patients with severe asthma.
    Article Snippet: INTRODUCTION The fibrocytes are bone marrow–derived mesenchymal progenitor cells that may contribute to the development of irreversible structural alterations in asthmatic airways by promoting subepithelial fibrosis and by expanding the population of contractile cells in the bronchial wall.. Increased numbers of fibrocytes have been detected in the peripheral blood and in the airways of asthmatic patients, particularly in those with a transient acute exacerbation of asthma and in subjects with chronically severe/treatmentrefractory disease.. The CD34þCD45ROþ fibrocytes that accumulate in the bronchial mucosa of asthmatic individuals actively synthesize new collagen and express the contractile protein a-smooth muscle actin (a-SMA).

    Article Title: CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex
    Article Snippet: .. Thymocyte suspensions were subjected to density centrifugation using Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS), and Early T-cell progenitors (ETPs) were then isolated by immunomagnetic cell sorting using the Dynal CD34 Progenitor Cell Selection System (Life Technologies). ..

    Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice
    Article Snippet: .. CD34 + cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal CD34 progenitor cell selection system (Invitrogen) according to the manufacturer's instructions. ..

    Article Title: Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias
    Article Snippet: .. CD34 + cells were then isolated with immunomagnetic microbeads and the Dynal CD34 progenitor cell selection system (Dynal Biotechnologies), according to the manufacturer's instructions. ..

    Article Title: Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias
    Article Snippet: .. CD34 + cells were isolated from mononuclear cells using immunomagnetic microbeads and the Dynal CD34 progenitor cell selection system (Dynal Biotechnologies, Oslo, Norway). .. B-lymphocytes were purified by negative selection using the RosetteSep human B cell enrichment cocktail (STEMCELL Technologies, Grenoble, France).

    Isolation:

    Article Title: Biochemical and Parasitological Studies on the Effect of hUCB-Selected CD34 + Progenitor/Stem Cells in Mice Infected with Schistosoma mansoni
    Article Snippet: After Ficoll-paque gradient separation, collected hUCB MNCs were washed twice in Dulbecco’s PBS. .. Isolation of the positively selected CD34 cells was then carried out using immunomagnetic separation technique by immune beads of Dynal CD34 + progenitor cell selection system (MACS ® ) following the procedure protocol according to the manufacturer’s recommendations. ..

    Article Title: The NOTCH1/CD44 axis drives pathogenesis in a T cell acute lymphoblastic leukemia model
    Article Snippet: .. ETPs were isolated as described ( 19 ) from thymocyte suspensions obtained by centrifugation on Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS) using the Dynal CD34 Progenitor Cell Selection System (Life Technologies). .. HSPCs were obtained from Ficoll-Hypaque–purified CB samples by AutoMACS immunomagnetic sorting using the CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec).

    Article Title: CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex
    Article Snippet: .. Thymocyte suspensions were subjected to density centrifugation using Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS), and Early T-cell progenitors (ETPs) were then isolated by immunomagnetic cell sorting using the Dynal CD34 Progenitor Cell Selection System (Life Technologies). ..

    Article Title: Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias
    Article Snippet: .. CD34 + cells were then isolated with immunomagnetic microbeads and the Dynal CD34 progenitor cell selection system (Dynal Biotechnologies), according to the manufacturer's instructions. ..

    Article Title: Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias
    Article Snippet: .. CD34 + cells were isolated from mononuclear cells using immunomagnetic microbeads and the Dynal CD34 progenitor cell selection system (Dynal Biotechnologies, Oslo, Norway). .. B-lymphocytes were purified by negative selection using the RosetteSep human B cell enrichment cocktail (STEMCELL Technologies, Grenoble, France).

    Immunomagnetic Separation:

    Article Title: Biochemical and Parasitological Studies on the Effect of hUCB-Selected CD34 + Progenitor/Stem Cells in Mice Infected with Schistosoma mansoni
    Article Snippet: After Ficoll-paque gradient separation, collected hUCB MNCs were washed twice in Dulbecco’s PBS. .. Isolation of the positively selected CD34 cells was then carried out using immunomagnetic separation technique by immune beads of Dynal CD34 + progenitor cell selection system (MACS ® ) following the procedure protocol according to the manufacturer’s recommendations. ..

    Magnetic Cell Separation:

    Article Title: Biochemical and Parasitological Studies on the Effect of hUCB-Selected CD34 + Progenitor/Stem Cells in Mice Infected with Schistosoma mansoni
    Article Snippet: After Ficoll-paque gradient separation, collected hUCB MNCs were washed twice in Dulbecco’s PBS. .. Isolation of the positively selected CD34 cells was then carried out using immunomagnetic separation technique by immune beads of Dynal CD34 + progenitor cell selection system (MACS ® ) following the procedure protocol according to the manufacturer’s recommendations. ..

    Centrifugation:

    Article Title: The NOTCH1/CD44 axis drives pathogenesis in a T cell acute lymphoblastic leukemia model
    Article Snippet: .. ETPs were isolated as described ( 19 ) from thymocyte suspensions obtained by centrifugation on Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS) using the Dynal CD34 Progenitor Cell Selection System (Life Technologies). .. HSPCs were obtained from Ficoll-Hypaque–purified CB samples by AutoMACS immunomagnetic sorting using the CD34 Progenitor Cell Isolation Kit (Miltenyi Biotec).

    Article Title: CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex
    Article Snippet: .. Thymocyte suspensions were subjected to density centrifugation using Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS), and Early T-cell progenitors (ETPs) were then isolated by immunomagnetic cell sorting using the Dynal CD34 Progenitor Cell Selection System (Life Technologies). ..



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    Image Search Results


    Specification of monoclonal antibodies

    Journal: Journal of Hematology & Oncology

    Article Title: Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones

    doi: 10.1186/s13045-016-0292-z

    Figure Lengend Snippet: Specification of monoclonal antibodies

    Article Snippet: MNC were washed twice with MACS CD34+ buffer, and CD34+ progenitor cells were purified using a MACS CD34+ progenitor cell selection isolation kit (Miltenlyi Biotech, Germany) according to the manufacturer’s instructions.

    Techniques:

    Gating strategy in newly diagnosed AML to identify CD34+/CD38−/CD123+ cells

    Journal: Journal of Hematology & Oncology

    Article Title: Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones

    doi: 10.1186/s13045-016-0292-z

    Figure Lengend Snippet: Gating strategy in newly diagnosed AML to identify CD34+/CD38−/CD123+ cells

    Article Snippet: MNC were washed twice with MACS CD34+ buffer, and CD34+ progenitor cells were purified using a MACS CD34+ progenitor cell selection isolation kit (Miltenlyi Biotech, Germany) according to the manufacturer’s instructions.

    Techniques:

    Gating strategy in normal BM to identify CD34+/CD38−/CD123+ cells

    Journal: Journal of Hematology & Oncology

    Article Title: Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones

    doi: 10.1186/s13045-016-0292-z

    Figure Lengend Snippet: Gating strategy in normal BM to identify CD34+/CD38−/CD123+ cells

    Article Snippet: MNC were washed twice with MACS CD34+ buffer, and CD34+ progenitor cells were purified using a MACS CD34+ progenitor cell selection isolation kit (Miltenlyi Biotech, Germany) according to the manufacturer’s instructions.

    Techniques:

    Summary of results from sorting and immunomagnetic cell selection  MACS

    Journal: Journal of Hematology & Oncology

    Article Title: Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones

    doi: 10.1186/s13045-016-0292-z

    Figure Lengend Snippet: Summary of results from sorting and immunomagnetic cell selection MACS

    Article Snippet: MNC were washed twice with MACS CD34+ buffer, and CD34+ progenitor cells were purified using a MACS CD34+ progenitor cell selection isolation kit (Miltenlyi Biotech, Germany) according to the manufacturer’s instructions.

    Techniques: Selection, Biomarker Discovery, Sequencing

    FLT3/ITD mutations in the BM cells of 7 AML patients. a CD34+/CD38−/CD123+ and CD34+/CD38−/CD123− cells from different patient samples were isolated by sorting (sorting results displayed in Table ). For each of the seven samples, genomic DNA was isolated from the ( 1 ) CD34+/CD38−/CD123−, ( 2 ) CD34+/CD38−/CD123+ cells, and ( 3 ) unsorted cells at diagnosis (a vertical line was inserted to indicate that diagnosis samples were done on separate gels). b The sequences of the FLT3/ITD mutations from the two samples (patients 3 and 4) are shown. These sequences, obtained from the DNA of CD34+/CD38−/CD123+ cells, matched the sequences of the mutations present in the pre-sorted samples. bp indicates base pair. Boldface sequence refers to the inserted, duplicated material

    Journal: Journal of Hematology & Oncology

    Article Title: Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones

    doi: 10.1186/s13045-016-0292-z

    Figure Lengend Snippet: FLT3/ITD mutations in the BM cells of 7 AML patients. a CD34+/CD38−/CD123+ and CD34+/CD38−/CD123− cells from different patient samples were isolated by sorting (sorting results displayed in Table ). For each of the seven samples, genomic DNA was isolated from the ( 1 ) CD34+/CD38−/CD123−, ( 2 ) CD34+/CD38−/CD123+ cells, and ( 3 ) unsorted cells at diagnosis (a vertical line was inserted to indicate that diagnosis samples were done on separate gels). b The sequences of the FLT3/ITD mutations from the two samples (patients 3 and 4) are shown. These sequences, obtained from the DNA of CD34+/CD38−/CD123+ cells, matched the sequences of the mutations present in the pre-sorted samples. bp indicates base pair. Boldface sequence refers to the inserted, duplicated material

    Article Snippet: MNC were washed twice with MACS CD34+ buffer, and CD34+ progenitor cells were purified using a MACS CD34+ progenitor cell selection isolation kit (Miltenlyi Biotech, Germany) according to the manufacturer’s instructions.

    Techniques: Isolation, Biomarker Discovery, Sequencing

    A. A representative histogram and dot plots showing CD34-expression after purification from cord blood depicted as fluorescence intensity values and percentage of CD34+ cells compared to the corresponding isotype control, respectively. B. The morphology and culture procedure for CD34+ cells after purification, seven days in expansion media, three days in medium and another three days in EGM-2 on fibronectin coated dishes. The fold expansion of cells represent mean and SD of n = 9.

    Journal: PLoS ONE

    Article Title: Successful In Vitro Expansion and Differentiation of Cord Blood Derived CD34+ Cells into Early Endothelial Progenitor Cells Reveals Highly Differential Gene Expression

    doi: 10.1371/journal.pone.0023210

    Figure Lengend Snippet: A. A representative histogram and dot plots showing CD34-expression after purification from cord blood depicted as fluorescence intensity values and percentage of CD34+ cells compared to the corresponding isotype control, respectively. B. The morphology and culture procedure for CD34+ cells after purification, seven days in expansion media, three days in medium and another three days in EGM-2 on fibronectin coated dishes. The fold expansion of cells represent mean and SD of n = 9.

    Article Snippet: The washed MNCs were then subjected to magnetic beads-based selection of CD34+ cells using Dynal® CD34 Progenitor Cells Selection System (Invitrogen, Oslo, Norway) following the manufacturer's recommendation.

    Techniques: Expressing, Purification, Fluorescence

    A. Binding of ulex-lectin and uptake of acetylated LDL (AcLDL) on EPCs visualised with fluorescence microscopy and flow cytometry using FITC-labelled ulex-lectin and Dil-labelled acetylated LDL, respectively. B. Summary of median fluorescence intensity for CD34-FITC, CD61-FITC, CD144-PE, CD309-PE, CD45-FITC and CD18-FITC of five donors expressed as mean and SD compensated for respective isotype control. C. Representative histograms comparing fluorescence intensity on early EPCs and HUVECs of the cell surface markers for CD34, CD61, CD144, CD309, CD45 and CD18 (blue line) and corresponding isotype control (red line).

    Journal: PLoS ONE

    Article Title: Successful In Vitro Expansion and Differentiation of Cord Blood Derived CD34+ Cells into Early Endothelial Progenitor Cells Reveals Highly Differential Gene Expression

    doi: 10.1371/journal.pone.0023210

    Figure Lengend Snippet: A. Binding of ulex-lectin and uptake of acetylated LDL (AcLDL) on EPCs visualised with fluorescence microscopy and flow cytometry using FITC-labelled ulex-lectin and Dil-labelled acetylated LDL, respectively. B. Summary of median fluorescence intensity for CD34-FITC, CD61-FITC, CD144-PE, CD309-PE, CD45-FITC and CD18-FITC of five donors expressed as mean and SD compensated for respective isotype control. C. Representative histograms comparing fluorescence intensity on early EPCs and HUVECs of the cell surface markers for CD34, CD61, CD144, CD309, CD45 and CD18 (blue line) and corresponding isotype control (red line).

    Article Snippet: The washed MNCs were then subjected to magnetic beads-based selection of CD34+ cells using Dynal® CD34 Progenitor Cells Selection System (Invitrogen, Oslo, Norway) following the manufacturer's recommendation.

    Techniques: Binding Assay, Fluorescence, Microscopy, Flow Cytometry

    A. A representative colony forming unit (CFU-Hill) visualised with giemsa staining and characterised with binding of FITC-labelled ulex-lectin and uptake of Dil-labelled acetylated LDL. B. Representative images of capillary network formed in a endothelial tube formation matrigel assay by HUVECs alone or HUVECs co-cultured with early EPCs. C Early EPCs pre-stained with cell tracker green (arrows) traced along the tubuli and in the branching area of capillary network D. The bar graph shows the total number of branching points (mean and SD, n = 3) and the box plot show the length of tubuli (dotted line represents the mean and the solid line represents the median, n = 488) in the endothelial tube formation matrigel with HUVECs alone or HUVECs co-cultured with early EPCs.

    Journal: PLoS ONE

    Article Title: Successful In Vitro Expansion and Differentiation of Cord Blood Derived CD34+ Cells into Early Endothelial Progenitor Cells Reveals Highly Differential Gene Expression

    doi: 10.1371/journal.pone.0023210

    Figure Lengend Snippet: A. A representative colony forming unit (CFU-Hill) visualised with giemsa staining and characterised with binding of FITC-labelled ulex-lectin and uptake of Dil-labelled acetylated LDL. B. Representative images of capillary network formed in a endothelial tube formation matrigel assay by HUVECs alone or HUVECs co-cultured with early EPCs. C Early EPCs pre-stained with cell tracker green (arrows) traced along the tubuli and in the branching area of capillary network D. The bar graph shows the total number of branching points (mean and SD, n = 3) and the box plot show the length of tubuli (dotted line represents the mean and the solid line represents the median, n = 488) in the endothelial tube formation matrigel with HUVECs alone or HUVECs co-cultured with early EPCs.

    Article Snippet: The washed MNCs were then subjected to magnetic beads-based selection of CD34+ cells using Dynal® CD34 Progenitor Cells Selection System (Invitrogen, Oslo, Norway) following the manufacturer's recommendation.

    Techniques: Staining, Binding Assay, Matrigel Assay, Cell Culture

    Fetal thymus organ cultures (FTOC) of shCD3δ-3 vs. shNT-transduced human T-cell progenitors. Top and middle: mouse embryo thymic lobes were seeded with shCD3δ-3 or shNT-transduced CD34 h i early T-cell progenitors from human thymus and cultured for up to 5 weeks. T-cell generation was analyzed on electronically gated GFP + transduced cells recovered from different lobes pooled at the indicated time points in two independent experiments. Bottom: representative (FTOC 2) flow cytometry analysis of CD3 vs. αβTCR expression on electronically gated GFP + cell progenies at the indicated days. Numbers in biparametric histograms indicate percentages of CD3 + αβTCR + (αβ T cells), CD3 – /low αβTCR – (including pre-TCR + pre-T cells) and CD3 + αβTCR – (γδ T cells). Percentages of γδ T cells were independently confirmed by CD3 vs. γδTCR expression analysis. Numbers in monoparametric histograms indicate MFI values for each indicated subset.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex

    doi: 10.3389/fcell.2021.608490

    Figure Lengend Snippet: Fetal thymus organ cultures (FTOC) of shCD3δ-3 vs. shNT-transduced human T-cell progenitors. Top and middle: mouse embryo thymic lobes were seeded with shCD3δ-3 or shNT-transduced CD34 h i early T-cell progenitors from human thymus and cultured for up to 5 weeks. T-cell generation was analyzed on electronically gated GFP + transduced cells recovered from different lobes pooled at the indicated time points in two independent experiments. Bottom: representative (FTOC 2) flow cytometry analysis of CD3 vs. αβTCR expression on electronically gated GFP + cell progenies at the indicated days. Numbers in biparametric histograms indicate percentages of CD3 + αβTCR + (αβ T cells), CD3 – /low αβTCR – (including pre-TCR + pre-T cells) and CD3 + αβTCR – (γδ T cells). Percentages of γδ T cells were independently confirmed by CD3 vs. γδTCR expression analysis. Numbers in monoparametric histograms indicate MFI values for each indicated subset.

    Article Snippet: Thymocyte suspensions were subjected to density centrifugation using Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS), and Early T-cell progenitors (ETPs) were then isolated by immunomagnetic cell sorting using the Dynal CD34 Progenitor Cell Selection System (Life Technologies).

    Techniques: Cell Culture, Flow Cytometry, Expressing

    The purity of enriched cells were determined by flow cytometry, after isolation cells were incubated with fluorescein isothiocyanate (FITC) conjugated mouse antihuman CD34 monoclonal antibody. The purity of the CD34+ cells was between 81 and 93.4% as seen in sample above. (Control refers to the group incubated only with serum free medium (SFC)). (UL: Upper Left; UR: Upper Right; LL: Lower Left and LR: Lower Right)

    Journal: Cytotechnology

    Article Title: Protective effects of cytokine combinations against the apoptotic activity of glucocorticoids on CD34 + hematopoietic stem/progenitor cells

    doi: 10.1007/s10616-018-0265-x

    Figure Lengend Snippet: The purity of enriched cells were determined by flow cytometry, after isolation cells were incubated with fluorescein isothiocyanate (FITC) conjugated mouse antihuman CD34 monoclonal antibody. The purity of the CD34+ cells was between 81 and 93.4% as seen in sample above. (Control refers to the group incubated only with serum free medium (SFC)). (UL: Upper Left; UR: Upper Right; LL: Lower Left and LR: Lower Right)

    Article Snippet: Enrichment and purification of CD34 + HSPCs A CD34 + HSPC population was collected from human peripheral blood buffy coat using an immunomagnetic positive selection method with the Dynal CD34 Human Progenitor Cell Selection Kit (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions.

    Techniques: Flow Cytometry, Isolation, Incubation, Control

    Determining degree of peroxidase reaction in CD34+ hematopoietic cells. (+++) cells stained at intensity 3, (++) cells stained at intensity 2, (+) cells stained at intensity 1, (−) no reaction

    Journal: Cytotechnology

    Article Title: Protective effects of cytokine combinations against the apoptotic activity of glucocorticoids on CD34 + hematopoietic stem/progenitor cells

    doi: 10.1007/s10616-018-0265-x

    Figure Lengend Snippet: Determining degree of peroxidase reaction in CD34+ hematopoietic cells. (+++) cells stained at intensity 3, (++) cells stained at intensity 2, (+) cells stained at intensity 1, (−) no reaction

    Article Snippet: Enrichment and purification of CD34 + HSPCs A CD34 + HSPC population was collected from human peripheral blood buffy coat using an immunomagnetic positive selection method with the Dynal CD34 Human Progenitor Cell Selection Kit (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions.

    Techniques: Staining