cd34 progenitor cells selection system (Thermo Fisher)
Structured Review

Cd34 Progenitor Cells Selection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd34+progenitor+cell+selection+system/pmc03155543-43-15-20
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Successful In Vitro Expansion and Differentiation of Cord Blood Derived CD34+ Cells into Early Endothelial Progenitor Cells Reveals Highly Differential Gene Expression"
Article Title: Successful In Vitro Expansion and Differentiation of Cord Blood Derived CD34+ Cells into Early Endothelial Progenitor Cells Reveals Highly Differential Gene Expression
Journal: PLoS ONE
doi: 10.1371/journal.pone.0023210
Figure Legend Snippet: A. A representative histogram and dot plots showing CD34-expression after purification from cord blood depicted as fluorescence intensity values and percentage of CD34+ cells compared to the corresponding isotype control, respectively. B. The morphology and culture procedure for CD34+ cells after purification, seven days in expansion media, three days in medium and another three days in EGM-2 on fibronectin coated dishes. The fold expansion of cells represent mean and SD of n = 9.
Techniques Used: Expressing, Purification, Fluorescence
Figure Legend Snippet: A. Binding of ulex-lectin and uptake of acetylated LDL (AcLDL) on EPCs visualised with fluorescence microscopy and flow cytometry using FITC-labelled ulex-lectin and Dil-labelled acetylated LDL, respectively. B. Summary of median fluorescence intensity for CD34-FITC, CD61-FITC, CD144-PE, CD309-PE, CD45-FITC and CD18-FITC of five donors expressed as mean and SD compensated for respective isotype control. C. Representative histograms comparing fluorescence intensity on early EPCs and HUVECs of the cell surface markers for CD34, CD61, CD144, CD309, CD45 and CD18 (blue line) and corresponding isotype control (red line).
Techniques Used: Binding Assay, Fluorescence, Microscopy, Flow Cytometry
Figure Legend Snippet: A. A representative colony forming unit (CFU-Hill) visualised with giemsa staining and characterised with binding of FITC-labelled ulex-lectin and uptake of Dil-labelled acetylated LDL. B. Representative images of capillary network formed in a endothelial tube formation matrigel assay by HUVECs alone or HUVECs co-cultured with early EPCs. C Early EPCs pre-stained with cell tracker green (arrows) traced along the tubuli and in the branching area of capillary network D. The bar graph shows the total number of branching points (mean and SD, n = 3) and the box plot show the length of tubuli (dotted line represents the mean and the solid line represents the median, n = 488) in the endothelial tube formation matrigel with HUVECs alone or HUVECs co-cultured with early EPCs.
Techniques Used: Staining, Binding Assay, Matrigel Assay, Cell Culture
Related Articles
Purification:Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice Article Snippet: .. CD34 cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice Article Snippet: .. CD34 + cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal Derivative Assay:Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice Article Snippet: .. CD34 cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice Article Snippet: .. CD34 + cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal FACS:Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice Article Snippet: .. CD34 cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal Article Title: CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex Article Snippet: .. Thymocyte suspensions were subjected to density centrifugation using Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS), and Early T-cell progenitors (ETPs) were then isolated by immunomagnetic cell sorting using the Dynal Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice Article Snippet: .. CD34 + cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal Selection:Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice Article Snippet: .. CD34 cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal Article Title: Biochemical and Parasitological Studies on the Effect of hUCB-Selected CD34 + Progenitor/Stem Cells in Mice Infected with Schistosoma mansoni Article Snippet: After Ficoll-paque gradient separation, collected hUCB MNCs were washed twice in Dulbecco’s PBS. .. Isolation of the positively selected CD34 cells was then carried out using immunomagnetic separation technique by immune beads of Dynal Article Title: The NOTCH1/CD44 axis drives pathogenesis in a T cell acute lymphoblastic leukemia model Article Snippet: .. ETPs were isolated as described ( 19 ) from thymocyte suspensions obtained by centrifugation on Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS) using the Dynal Article Title: The C-C motif chemokine ligands CCL5, CCL11, and CCL24 induce the migration of circulating fibrocytes from patients with severe asthma. Article Snippet: INTRODUCTION The fibrocytes are bone marrow–derived mesenchymal progenitor cells that may contribute to the development of irreversible structural alterations in asthmatic airways by promoting subepithelial fibrosis and by expanding the population of contractile cells in the bronchial wall.. Increased numbers of fibrocytes have been detected in the peripheral blood and in the airways of asthmatic patients, particularly in those with a transient acute exacerbation of asthma and in subjects with chronically severe/treatmentrefractory disease.. The CD34þCD45ROþ fibrocytes that accumulate in the bronchial mucosa of asthmatic individuals actively synthesize new collagen and express the contractile protein a-smooth muscle actin (a-SMA). Article Title: CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex Article Snippet: .. Thymocyte suspensions were subjected to density centrifugation using Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS), and Early T-cell progenitors (ETPs) were then isolated by immunomagnetic cell sorting using the Dynal Article Title: T Cells Modulate Epstein-Barr Virus Latency Phenotypes during Infection of Humanized Mice Article Snippet: .. CD34 + cells were purified from cord blood derived from healthy full-term newborns by a magnetically activated cell sorting cell separation system using anti-CD34 microbeads (Miltenyi Biotech) or a Dynal Article Title: Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias Article Snippet: .. CD34 + cells were then isolated with immunomagnetic microbeads and the Dynal Article Title: Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias Article Snippet: .. CD34 + cells were isolated from mononuclear cells using immunomagnetic microbeads and the Dynal Isolation:Article Title: Biochemical and Parasitological Studies on the Effect of hUCB-Selected CD34 + Progenitor/Stem Cells in Mice Infected with Schistosoma mansoni Article Snippet: After Ficoll-paque gradient separation, collected hUCB MNCs were washed twice in Dulbecco’s PBS. .. Isolation of the positively selected CD34 cells was then carried out using immunomagnetic separation technique by immune beads of Dynal Article Title: The NOTCH1/CD44 axis drives pathogenesis in a T cell acute lymphoblastic leukemia model Article Snippet: .. ETPs were isolated as described ( 19 ) from thymocyte suspensions obtained by centrifugation on Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS) using the Dynal Article Title: CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex Article Snippet: .. Thymocyte suspensions were subjected to density centrifugation using Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS), and Early T-cell progenitors (ETPs) were then isolated by immunomagnetic cell sorting using the Dynal Article Title: Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias Article Snippet: .. CD34 + cells were then isolated with immunomagnetic microbeads and the Dynal Article Title: Chromatin Redistribution of the DEK Oncoprotein Represses hTERT Transcription in Leukemias Article Snippet: .. CD34 + cells were isolated from mononuclear cells using immunomagnetic microbeads and the Dynal Immunomagnetic Separation:Article Title: Biochemical and Parasitological Studies on the Effect of hUCB-Selected CD34 + Progenitor/Stem Cells in Mice Infected with Schistosoma mansoni Article Snippet: After Ficoll-paque gradient separation, collected hUCB MNCs were washed twice in Dulbecco’s PBS. .. Isolation of the positively selected CD34 cells was then carried out using immunomagnetic separation technique by immune beads of Dynal Magnetic Cell Separation:Article Title: Biochemical and Parasitological Studies on the Effect of hUCB-Selected CD34 + Progenitor/Stem Cells in Mice Infected with Schistosoma mansoni Article Snippet: After Ficoll-paque gradient separation, collected hUCB MNCs were washed twice in Dulbecco’s PBS. .. Isolation of the positively selected CD34 cells was then carried out using immunomagnetic separation technique by immune beads of Dynal Centrifugation:Article Title: The NOTCH1/CD44 axis drives pathogenesis in a T cell acute lymphoblastic leukemia model Article Snippet: .. ETPs were isolated as described ( 19 ) from thymocyte suspensions obtained by centrifugation on Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS) using the Dynal Article Title: CD3G or CD3D Knockdown in Mature, but Not Immature, T Lymphocytes Similarly Cripples the Human TCRαβ Complex Article Snippet: .. Thymocyte suspensions were subjected to density centrifugation using Ficoll-Hypaque (Lymphoprep, Axis-Shield PoC AS), and Early T-cell progenitors (ETPs) were then isolated by immunomagnetic cell sorting using the Dynal |


